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Journal: MedComm
Article Title: Dormant Metastases Exhibit a Unique Phenotype Primarily Promoted by the Ch25h Gene and Are Maintained in Dormancy by T Lymphocytes
doi: 10.1002/mco2.70437
Figure Lengend Snippet: Genes differentially expressed in the dormant metastasis group. Heat maps are presented to illustrate the differential expression data between the three metastatic groups obtained from RNA‐Seq (A), or RT‐qPCR in genes involved in the cholesterol synthesis pathway (B), in chemokine genes (C), and in surface marker genes (D). Only genes that displayed a greater than twofold difference in expression (log2>1) with a p ‐value less than 0.001 were identified and plotted in the heatmap. The expression values are presented as log2 values and are scaled by gene. In RT‐qPCR assays, the expression levels of the genes of interest were determined with respect to the levels of the β‐actin and GAPDH housekeeping genes. The data for the overt‐met group were set to 1. The values represent the means of three independent experiments performed in duplicate. The statistical analysis was performed using ANOVA, followed by Tukey's post hoc test.
Article Snippet: The
Techniques: Quantitative Proteomics, RNA Sequencing, Quantitative RT-PCR, Marker, Expressing
Journal: MedComm
Article Title: Dormant Metastases Exhibit a Unique Phenotype Primarily Promoted by the Ch25h Gene and Are Maintained in Dormancy by T Lymphocytes
doi: 10.1002/mco2.70437
Figure Lengend Snippet: MicroRNAs that are differentially expressed in the dormant metastasis group. (A) Heat map is presented to illustrate the differential expression data between the three metastatic groups. (B) miRNA enrichment analysis of miRNAs differentially expressed in dormant‐met group (miEAA). The expression of 834 miRNAs was measured using the ‘mouse miRNome qPCR arrays 18.0’ (Genecopoeia). Only miRNAs that displayed a greater than twofold difference ( p < 0.01) when comparing the dormant‐met group with the nude‐met and overt‐met groups were plotted. The expression levels of the miRNAs of interest were determined with respect to the levels of six housekeeping snRNAs (MK1‐MK6). The data for the overt‐met group were set to 1. The values are presented as the means of three independent experiments, each performed in duplicate. The statistical analysis was conducted using an ANOVA test, followed by a Tukey's post hoc test.
Article Snippet: The
Techniques: Quantitative Proteomics, Expressing